中国科学院上海光学精密机械研究所(简称:上海光机所)成立于1964年5月,是我国建立最早、规模最大的激光科学技术专业研究所。发展至今,已形成以探索现代光学重大基础及应用基础前沿、发展大型激光工程技术并开拓激光与光电子高技术应用为重点的综合性研究所。研究...
中国科学院上海光学精密机械研究所(简称:上海光机所)是我国建立最早、规模最大的激光专业研究所,成立于1964年,现已发展成为以探索现代光学重大基础及应用基础前沿研究、发展大型激光工程技术并开拓激光与光电子高技术应用为重点的综合性研究所。重...
上海光机所国际合作工作始终围绕上海光机所的主责主业,以服务重大任务和国家需求为牵引,强化目标导向,注重内外集成协同,加强重大国际合作任务的谋划。坚持“战略布局,需求牵引,技术引领,合作共赢”的原则,基于科技部授予的国家国际科技合作基地及本单位学科技术优势,围绕“一带一路”国家倡议,深化拓展与发达国家实质性合作,夯实海外机构建设,积极培育和发起国际大科学计划,加强国际组织任职推荐,组织相关国际会议等,汇聚各类国际人才,建立以“平台-人才-项目-组织”合作模式,融入全球创新合作网络,助力上海光机所成为国际一流科研机构。上海光机所国际合作一直得到所领导的高度重视,历届所长亲自主管国际合作。1972年,上海光机所接待诺贝尔奖的美籍华裔科学家杨振宁,标志着我所第一次对外开放。2007年,被科技部首批授予“科技部国际科技合作基地”。2016年,科技部首次对全国2006-2008年间认定的113家国际合作基地进行了评估,上海光机所获评“优秀”。2021年,科技部首次对全国719家国际合作基地进行了评估,上海光机所持续获评“ 优秀”。王岐山副主席到上海光机所视察时,对上海光机所近几年取得的系列科技成果,以及重大国际合作项目“中以高功...
作为我国建立最早、规模最大的激光科学技术专业研究所,和首批上海市科普教育基地之一,中国科学院上海光学精密机械研究所(简称:上海光机所)在致力于科技创新的同时,十分重视科普工作。多年来,上海光机所借助科研院所强大的科普资源优势,围绕光学与激光科学技术,积极开展公众开放日、科普讲座、科技课堂、科普作品创...
报告人: | 刘晓红 博士,中科院生物物理所 | |
报告题目: |
Metalloprotein design using genetic code expansion | |
报告时间: | 2019年1月11日(周五) 12:30-13:30 | |
报告地点: | 1号楼多功能厅 |
报告简介 | 议题海报 | 本期照片 | 本期议题简讯 |
Abstract Heme copper oxidase (HCO), which can reduce oxygen to water, is an indispensable substance in bioenergy production. The large molecular weight, difficult expression and spectral study of HCO are the bottlenecks restricting its mechanical research and application. Through the genetic incorporation of the Tyr-His ligand and CuB site into myoglobin, we recapitulated important features of HCO into this small soluble protein, which exhibits selective O2 reduction activity while generating less than 6% ROS, at more than 1000 turnovers. These results support that Tyr-His crosslink is indeed important for HCO function, and creates the exciting opportunity to rapidly evolve better HCO model proteins to achieve higher activity and selectivity, which may be suitable as alternatives to precious metal catalyst in fuel cells. By engineering more favorable electrostatic interactions between a functional oxidase model designed in sperm whale myoglobin and its native redox partner, cyt b5, resulting in a 400-fold electron transfer (ET) rate enhancement, with an O2 reduction rate (52 s-1) comparable to that of a native cytochrome (cyt) cbb3 oxidase (50 s-1) under identical conditions. Achieving high activity equivalent to that of native enzymes in a designed metalloenzyme offers deeper insight into the roles of tunable processes such as ET in oxidase activity and enzymatic function and may extend into applications such as more efficient oxygen reduction reaction catalysts for biofuel cells. Recently, based on our previous works, with genetic encode technology, we have developed a photosensitizer protein (PSP) by incorporating an unnatural amino acid (Bpa) containing a benzophenone side chain into chromophore of fluorescent protein. When ligated with a CO2 reduction catalyst, the PSP can be used as a photoredox catalyst for CO2 reduction. Compare with small photoredox molecules, the photoredox protein has the following additional merits: 1. heavy-metal free; 2. can be easily introduced into various organisms; 3. having dramatically expanded abilities through rational design or directed evolution. |
Biography Xiaohong LIU/刘晓红 e-mail: liuxh@moon.ibp.ac.cn http://www.ibp.cas.cn/kydw/ibpqchhy/201312/t20131216_3999517.html The main interest is to design metalloenzymes that are easy to characterize, produce and optimize by using small, soluble protein scaffolds and genetic incorporation of non-natural amino acids. These metalloenzymes catalyze important reactions with efficiency/selectivity equal to or higher than natural systems. Education- Ph.D., Sep. 2002-Jul. 2005, Department of Chemistry, Tsinghua university, Organic Chemistry M.S., Sep. 1999-Jul. 2002, Chemistry and Life Science College, Tianjin Normal University, Physical Chemistry B.S., Sep. 1995-Jul. 1999, Chemistry and Life Science College, Tianjin Normal University, Physical Chemistry Work Experience- Jan.2015-Pre. Professor, Institute of Biophysics, CAS Jan.2012-Dec.2014 Associate professor, Institute of Biophysics, CAS May.2008-Dec.2011 Assistance Professor, Institute of Biophysics, CAS Aug.2005-Mar.2008 Postdoctoral Fellow, Teikyo University of Science and Technology, Yamanashi Prefecture, Japan Representative Publicaitions- 1) X.H. Liu, F.Y. Kang, C. Hu, L. Wang, Z Xu, D.D Zheng, J. Huang, Y. Lu, P.G. Schultz, J.Y. Wang. A genetically encoded photosensitizer protein facilitates the rational design of a miniature photocatalytic CO2 reducing enzyme. Nature Chemistry (2018) 10, 1201–1206. 2) Y. Yu1, C Cui1, X.H. Liu1, I. D. Petrik, J.Y. Wang, and Y. Lu. A Designed Metalloenzyme Achieving the Catalytic Rate of a Native Enzyme. Journal of the American Chemical Society, (2015) 137 (36), 11570–11573 3) X.H. Liu1, L Jiang1, J Li1, L Wang, Y Yu, Q Zhou, X Lv, W Gong, Y Lu, JY Wang. Significant Expansion of Fluorescent Protein Sensing Ability through the Genetic Incorporation of Superior Photo-Induced Electron-Transfer Quenchers Journal of the American Chemical Society, (2014)136(38), 13094-13097 4) X.H. Liu1, J.S. Li1, C. Hu, Q. Zhou, W. Zhang, M.R. Hu, J.Z. Zhou, J.Y. Wang. Significant Expansion of the Fluorescent Protein Chromophore through the Genetic Incorporation of a Metal-chelating Unnatural Amino Acid Angew Chem Intl Ed (2013) 52(18), 4805-4809 5) X.H. Liu1, J.S. Li1, J.S. Dong1, C. Hu, W.M. Gong, and J.Y. Wang. Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer Angewandte Chemie International Edition (2012) ;51(41):10261-5 6) X.H. Liu1, Y. Yu1, C. Hu, W. Zhang, Y. Lu, and J.Y. Wang. Significant Increase of oxidase activity through the genetic incorporation of a Tyrosine-Histidine cross-link in a Myoglobin model of Heme-Copper oxidase Angewandte Chemie International Edition (2012);51(18):4312-6 |
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